6XUO

mature recombinant horse NGF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	90
Detector technology	PIXEL
Collection date	2016-04-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	I 21 3
Unit cell lengths	108.490, 108.490, 108.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.245 - 2.803
R-factor	0.2238
Rwork	0.219
R-free	0.26610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sg1
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.710	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.072	0.858
Rmeas	0.074	0.884
Rpim	0.018	0.212
Number of reflections	5381	776
<I/σ(I)>	24.8	3.6
Completeness [%]	100.0	100
Redundancy	17.5	17
CC(1/2)	1.000	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.11 M Zn acetate, 0.1 M MES (pH 6.5)