6XTE

Human karyopherin RanBP5 (isoform-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	87.091, 96.313, 156.979
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.490 - 2.270
R-factor	0.2019
Rwork	0.199
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w3u
RMSD bond length	0.009
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.540
High resolution limit [Å]	2.270	2.270
Rmerge	0.089	0.892
Rpim	0.046	0.452
Number of reflections	41396	2070
<I/σ(I)>	9.3	1.7
Completeness [%]	70.3	14.5
Redundancy	4.4	4.3
CC(1/2)	0.996	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM tri-sodium citrate pH 4.5, 12 % PEG 6000, 100 mM ammonium acetate