6XT1
The structure of the M60 catalytic domain from Clostridium perfringens ZmpC in complex the sialyl T antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-07 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.615, 100.930, 119.024 |
| Unit cell angles | 90.00, 95.91, 90.00 |
Refinement procedure
| Resolution | 39.070 - 2.490 |
| R-factor | 0.2368 |
| Rwork | 0.234 |
| R-free | 0.27970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xsz |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.702 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.540 |
| High resolution limit [Å] | 2.490 | 2.500 |
| Rmerge | 0.109 | 0.589 |
| Rpim | 0.051 | 0.317 |
| Number of reflections | 89691 | 4505 |
| <I/σ(I)> | 15.3 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.2 | 4 |
| CC(1/2) | 0.988 | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 291 | 7.6% Tascimate, 19% PEG 3350 |
