6XSW
Structure of the Notch3 NRR in complex with an antibody Fab Fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2012-07-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 133.652, 158.951, 159.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 112.660 - 2.980 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xgp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 159.690 | 3.140 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.189 | 1.099 |
| Rpim | 0.079 | 0.456 |
| Number of reflections | 70289 | 10115 |
| <I/σ(I)> | 10 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM sodium Citrate pH 5.5, 12 % PEG 6000 |
