6XQ8

Receptor for Advanced Glycation End Products VC1 domain in complex with Fragments 1 & 11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-15
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 62
Unit cell lengths	102.017, 102.017, 102.779
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.690 - 1.820
R-factor	0.1994
Rwork	0.198
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lp4
RMSD bond length	0.019
RMSD bond angle	1.504
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.890
High resolution limit [Å]	1.820	1.820
Rmeas	0.056	0.309
Rpim	0.019	0.107
Number of reflections	107292	10717
<I/σ(I)>	19.22	5.05
Completeness [%]	99.9
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	2.5 M NaOAc (pH 7.5)