6XPR

Human antibody D2 H1-1/H3-1 H3 in complex with the influenza hemagglutinin head domain of A/Texas/50/2012(H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-10-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 3 2 1
Unit cell lengths	204.490, 204.490, 132.490
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.117 - 4.092
R-factor	0.2541
Rwork	0.252
R-free	0.29810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5W08 4y5v & 6E4X
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.120	4.238
High resolution limit [Å]	4.092	4.092
Rmerge	0.306	0.855
Rmeas	0.361	1.012
Rpim	0.190	0.533
Number of reflections	25192	8466
<I/σ(I)>	4.53	1.6
Completeness [%]	98.4	98.07
Redundancy	3.4	3.4
CC(1/2)	0.965	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291.15	0.1 M lithium sulfate, 30% v/v PEG400, 0.1 M trisodium citrate, pH 5.6