6XPC
Structure of human GGT1 in complex with full GSH molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 105.395, 124.679, 104.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.260 |
| R-factor | 0.1619 |
| Rwork | 0.159 |
| R-free | 0.21010 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4gdx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.301 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.390 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.080 | 0.620 |
| Number of reflections | 32583 | 4931 |
| <I/σ(I)> | 15.01 | 2.52 |
| Completeness [%] | 98.8 | 93.8 |
| Redundancy | 6.47 | 6.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 20-25%PEG3350, 0.1M ammonium cloride, 0.1M sodium cacodilate pH 6.0 |
