6XLW

Crystal structure of U2AF65 bound to AdML splice site sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	43.398, 62.731, 77.427
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.710 - 1.500
R-factor	0.1326
Rwork	0.131
R-free	0.15350
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008
RMSD bond angle	1.038
Data reduction software	XDS
Data scaling software	Aimless (0.5.8)
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.710	1.520
High resolution limit [Å]	1.500	1.500
Rmerge	0.053	0.098
Rpim	0.032	0.062
Number of reflections	33522	1417
<I/σ(I)>	22.3	9.9
Completeness [%]	97.0	85
Redundancy	6.9	6
CC(1/2)	0.998	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	1M Succinic Acid, 0.1M HEPES pH 7, 3% PEG MME 2000