6XLI
CRYSTAL STRUCTURE OF ANTI-TAU ANTIBODY PT3 Fab+pT212/pT217-TAU PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.240, 83.660, 166.870 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 36.159 - 2.000 |
| R-factor | 0.1863 |
| Rwork | 0.186 |
| R-free | 0.21330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i19 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.830 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.053 | 0.555 |
| Number of reflections | 116430 | 8607 |
| <I/σ(I)> | 13.09 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20% PEG 3350, 0.2 M NH4H2PO4 |
