6XL1
crystal structure of cA4-activated Card1(D294N)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 111.946, 111.946, 172.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.580 - 1.950 |
R-factor | 0.1791 |
Rwork | 0.178 |
R-free | 0.20160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wwx |
RMSD bond length | 0.008 |
RMSD bond angle | 0.843 |
Data reduction software | autoXDS |
Data scaling software | autoXDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.081 | 0.661 |
Rmeas | 0.083 | 0.684 |
Rpim | 0.021 | 0.174 |
Number of reflections | 80132 | 7872 |
<I/σ(I)> | 23.41 | 4.08 |
Completeness [%] | 99.9 | 99.94 |
Redundancy | 15 | 15.2 |
CC(1/2) | 0.998 | 0.936 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M HEPES 7.0, 30% (v/v) Jeffamine M-600 |