6XHW

Crystal structure of the A2058-unmethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.50A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	209.830, 448.310, 622.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	311.280 - 2.500
R-factor	0.2353
Rwork	0.233
R-free	0.28140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.004
RMSD bond angle	0.895
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	311.280	311.280	2.570
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.147	0.021	1.382
Rmeas	0.173	0.025	1.629
Total number of observations	6765671
Number of reflections	1940188	22765	135165
<I/σ(I)>	8.64	40.34	0.91
Completeness [%]	97.4	97	92.1
Redundancy	3.487	3.576	3.371
CC(1/2)	0.990	0.999	0.134

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME