6XFR

Metallo-beta-lactamase from Pontibacter korlensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95372
Spacegroup name	P 42 21 2
Unit cell lengths	97.941, 97.941, 103.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.245 - 2.608
R-factor	0.1792
Rwork	0.176
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c09
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	2.600
High resolution limit [Å]	2.600	2.600
Number of reflections	15978	15976
<I/σ(I)>	0.08
Completeness [%]	99.8
Redundancy	1
CC(1/2)	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	0.1 M Tris, 0.002 M ZnCl2, 20% w/v PEG 6000