6XF9
Crystal structure of KSHV ORF68
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.11589 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 134.987, 224.042, 192.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.540 - 2.220 |
| R-factor | 0.2227 |
| Rwork | 0.222 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ORF68-polyA |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.380 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.543 | 2.480 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Number of reflections | 189934 | 4570 |
| <I/σ(I)> | 19.4 | 1.7 |
| Completeness [%] | 95.3 | |
| Redundancy | 13.3 | |
| CC(1/2) | 0.999 | 0.565 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 310 mM CaCl2, 95 mM HEPES pH 7.5, 26.6% PEG-400, 5% glycerol |
