6XDS
Crystal structure of MBP-TREM2 Ig domain fusion with fragment, 2-((4-bromophenyl)amino)ethan-1-ol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.916360 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.840, 80.350, 103.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.440 - 1.466 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.20820 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BUSTER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.440 | 1.608 |
| High resolution limit [Å] | 1.466 | 1.466 |
| Number of reflections | 76286 | 3814 |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 72.3 | 15.5 |
| Redundancy | 6.6 | 6 |
| CC(1/2) | 0.999 | 0.572 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 100 mM BisTris pH 5.5, 100 mM ammonium acetate, 15% PEG4000, 15% glycerol |
