6XCP

Immune receptor complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	65.977, 116.788, 124.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.730 - 3.300
R-factor	0.2359
Rwork	0.234
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z7u
RMSD bond length	0.002
RMSD bond angle	0.500
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.730	3.418
High resolution limit [Å]	3.300	3.300
Rmerge	0.069	0.418
Rmeas	0.097	0.592
Rpim	0.069	0.418
Number of reflections	15005	1466
<I/σ(I)>	8.46	1.58
Completeness [%]	99.9	100
Redundancy	2
CC(1/2)	0.994	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	13% PEG 8000, 0.2M Potassium dihydrogen phosphate