6XB8

Adeno-Associated Virus Origin Binding Domain in complex with ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	110
Detector technology	CCD
Collection date	2011-03-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	170.180, 173.220, 173.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.860 - 3.300
R-factor	0.2019
Rwork	0.201
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5byg
RMSD bond length	0.005
RMSD bond angle	0.820
Data reduction software	MOSFLM
Data scaling software	pointless
Phasing software	PHENIX
Refinement software	PHENIX (1.18rc2_3794)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.450
High resolution limit [Å]	3.300	3.300
Rmerge	0.125	0.594
Number of reflections	38830	4683
<I/σ(I)>	15.5	5.42
Completeness [%]	99.9	99.9
Redundancy	10	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	10mM Na cacodylate, pH 6.5, 30% PEG400 and 0.2M LiCl