6X90

Structure of the guanine nucleotide exchange factor Sec12 bound to the small GTPase Sar1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	50.333, 93.620, 115.423
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.710 - 2.260
R-factor	0.2013
Rwork	0.199
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4h5i 1f6b
RMSD bond length	0.005
RMSD bond angle	0.784
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.710	2.330
High resolution limit [Å]	2.260	2.260
Rmerge	0.039	0.741
Rmeas	0.043	0.806
Rpim	0.017	0.313
Number of reflections	20215	1012
<I/σ(I)>	20.1	2.4
Completeness [%]	95.3	98.6
Redundancy	6.5	6.4
CC(1/2)	1.000	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	5mg/mL protein, 100mM MES pH6.5, 30% PEG 5000 MME