6X88
PDE6 chicken GAF domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 148.499, 148.499, 93.678 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.980 - 3.200 |
| R-factor | 0.214852968782 |
| Rwork | 0.210 |
| R-free | 0.30731 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.148 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.981 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.143 | 0.890 |
| Number of reflections | 19554 | 906 |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 86.0 | |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2 uL 8 mg/mL purified chicken cone GAFab protein + 2 uL well buffer (0.1 M HEPES sodium, pH 7.5, 21-27% PEG3350, 0.2 M ammonium sulfate, 20% glycerol) |
