6X5T
Human Alpha-1,6-fucosyltransferase (FUT8) bound to GDP and A3-Asn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 |
| Unit cell lengths | 151.690, 151.690, 474.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.998 - 2.470 |
| R-factor | 0.1684 |
| Rwork | 0.166 |
| R-free | 0.20030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6x5h |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.534 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.998 | 2.530 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmeas | 0.120 | 1.050 |
| Number of reflections | 219502 | 16316 |
| <I/σ(I)> | 10.7 | 1.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.1 | 5.3 |
| CC(1/2) | 0.997 | 0.505 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 10% PEG 3350, 0.2 M L-proline, 100 mM Hepes pH 7.2 |
