6X5S
Human Alpha-1,6-fucosyltransferase (FUT8) bound to GDP and A3'-Asn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 150.500, 150.500, 480.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.168 - 3.300 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.22370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6x5h |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.302 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.170 | 3.390 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmeas | 0.192 | 0.930 |
| Number of reflections | 44367 | 3352 |
| <I/σ(I)> | 7.9 | 1.8 |
| Completeness [%] | 89.4 | 93.6 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.990 | 0.689 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.2 M Lithium sulfate, 12 mM Nickel (II) chloride, 100 mM Tris pH 8.5 |
