6X2D

Crystal Structure of DNase I Domain of Ribonuclease E from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2020-03-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	40.614, 58.812, 47.046
Unit cell angles	90.00, 108.16, 90.00

Refinement procedure

Resolution	29.410 - 1.850
R-factor	0.2085
Rwork	0.207
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vrt
RMSD bond length	0.005
RMSD bond angle	1.292
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.088	0.759
Rmeas	0.097	0.833
Rpim	0.041	0.339
Number of reflections	17863	897
<I/σ(I)>	21.9	4.7
Completeness [%]	99.4	100
Redundancy	5.7	6
CC(1/2)	0.985	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	Protein: 7.9 mg/ml, 0.01M Tris pH 8.3; Screen: PACT (F3), 0.2M Sodium iodide, 0.1M Bis-Tris propane pH 6.5, 20% (w/v) PEG 3350