6WZQ

Structure of SARS-CoV-2 Nucleocapsid dimerization domain, P21 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	71.146, 43.546, 74.233
Unit cell angles	90.00, 94.87, 90.00

Refinement procedure

Resolution	73.970 - 1.450
R-factor	0.168
Rwork	0.167
R-free	0.18020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wxo
RMSD bond length	0.009
RMSD bond angle	0.922
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.000	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.058	0.802
Rmeas	0.063	0.877
Number of reflections	80163	3906
<I/σ(I)>	16	2
Completeness [%]	98.8
Redundancy	6.6
CC(1/2)	0.998	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	100 mM Tris-HCl pH 8.5, 50 mM Ammonium Sulfate, and 38% polyethylene glycol (PEG) 3350