6WX8

SOX2 bound to Importin-alpha 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-09-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	148.580, 118.990, 94.870
Unit cell angles	90.00, 128.77, 90.00

Refinement procedure

Resolution	29.100 - 2.300
R-factor	0.2215
Rwork	0.220
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bw9
RMSD bond length	0.002
RMSD bond angle	0.494
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.500	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	56880	4570
<I/σ(I)>	12.6
Completeness [%]	99.6
Redundancy	13
CC(1/2)	0.999	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.2M (NH4)2SO4, 0.1M HEPES pH 6.5