6WVD

Human JAGN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	90.774, 42.718, 111.782
Unit cell angles	90.00, 103.79, 90.00

Refinement procedure

Resolution	38.440 - 2.250
R-factor	0.1965
Rwork	0.195
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.290
High resolution limit [Å]	2.250	6.100	2.250
Rmerge	0.091	0.056	0.607
Rmeas	0.110	0.068	0.741
Rpim	0.062	0.037	0.418
Number of reflections	19195	1009	898
<I/σ(I)>	8.8
Completeness [%]	95.6	96	93.3
Redundancy	2.8	2.9	2.7
CC(1/2)		0.993	0.625

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		295	30% PEG400, 0.1 M Li2SO4, 0.1M NaCl, 0.1 M Tris pH 8.0