6WQX

Human PRPK-TPRKB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	66.856, 77.540, 100.413
Unit cell angles	90.00, 106.81, 90.00

Refinement procedure

Resolution	49.358 - 2.530
R-factor	0.2139
Rwork	0.211
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3enp 3en9
RMSD bond length	0.008
RMSD bond angle	0.992
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.360	2.640
High resolution limit [Å]	2.530	2.530
Rmerge	0.107	1.017
Number of reflections	32683	3987
<I/σ(I)>	11.1	2
Completeness [%]	99.1
Redundancy	5.1
CC(1/2)	0.997	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.1 M KCl, 0.1 M HEPES pH 7.5, 15% PEG 6000