6WP1
The Crystal Structure of Apo Domain-Swapped Trimer Q108K:K40L:T51K Variant of HCRBPII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-29 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.831, 78.466, 178.887 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.480 - 2.990 |
R-factor | 0.252 |
Rwork | 0.247 |
R-free | 0.30200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rct |
RMSD bond length | 0.002 |
RMSD bond angle | 0.578 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.480 | 3.030 |
High resolution limit [Å] | 2.986 | 2.986 |
Rmeas | 0.292 | 1.180 |
Number of reflections | 20546 | 1916 |
<I/σ(I)> | 13.1 | 2.3 |
Completeness [%] | 100.0 | |
Redundancy | 13.6 | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG4000, sodium acetate, ammonium acetate |