6WNO

Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 243244

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	61.010, 84.440, 90.910
Unit cell angles	90.73, 110.12, 103.09

Refinement procedure

Resolution	42.996 - 3.350
R-factor	0.2466
Rwork	0.245
R-free	0.28350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w53
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.996	42.996	3.440
High resolution limit [Å]	3.350	14.980	3.350
Rmerge	0.182	0.044	0.838
Rmeas	0.216	0.052	0.988
Total number of observations	81267
Number of reflections	23223	250	1674
<I/σ(I)>	6.9	24.66	1.59
Completeness [%]	97.7	94.3	97.4
Redundancy	3.499	3.4	3.47
CC(1/2)	0.989	0.997	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	0.2 M trimethylamine N-oxide, 0.1 M Tris chloride pH 8.5, 20% (w/v) PEG monomethyl ether 2,000