6WN4
Structural basis for the binding of monoclonal antibody 5D2 to the tryptophan-rich lipid-binding loop in lipoprotein lipase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.624, 67.745, 130.073 |
| Unit cell angles | 90.00, 94.37, 90.00 |
Refinement procedure
| Resolution | 29.264 - 2.800 |
| Rwork | 0.208 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d8j |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.263 |
| Data reduction software | HKL-2000 (v720) |
| Data scaling software | HKL-2000 (v720) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.264 | 2.840 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.120 | 0.692 |
| Rmeas | 0.133 | 0.769 |
| Rpim | 0.057 | 0.330 |
| Number of reflections | 23348 | 2178 |
| <I/σ(I)> | 10.4 | 1.9 |
| Completeness [%] | 99.3 | 93.72 |
| Redundancy | 5.1 | 5.08 |
| CC(1/2) | 0.995 | 0.729 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 16-22% PEG 3350, 250mM Sodium Thiocyanate |
