6WMW

GFRAL receptor bound with two antibody Fabs (3P10, 25M22)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 21 21 21
Unit cell lengths	52.498, 116.052, 227.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.700 - 2.910
R-factor	0.2251
Rwork	0.222
R-free	0.28970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vz4
RMSD bond length	0.008
RMSD bond angle	1.240
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.010
High resolution limit [Å]	2.900	6.270	2.910
Rmerge	0.099	0.044	0.787
Rmeas	0.108	0.047	0.864
Rpim	0.041	0.018	0.349
Number of reflections	31586	3416	3018
<I/σ(I)>	10.2
Completeness [%]	99.6	99.7	98.8
Redundancy	6.6	6.5	5.9
CC(1/2)		0.999	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	297	0.1 M IMIDAZOLE Ph 7.0 AND 12% (w/w) peg 20,000