6WMH
Next generation monomeric IgG4 Fc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-12 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.19499 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 102.974, 110.925, 87.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.300 |
| R-factor | 0.2028 |
| Rwork | 0.201 |
| R-free | 0.24310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hvw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.876 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.169 | 1.282 |
| Rpim | 0.069 | 0.528 |
| Number of reflections | 22417 | 2147 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.2 | 98.2 |
| Redundancy | 6.8 | 6.7 |
| CC(1/2) | 0.987 | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 295 | DL-Glutamic acid monohydrate, DL-Alanine, Glycine, DL-Lysine monohydrochloride, DL-Serine, Sodium HEPES, MOPS (acid), Ethylene glycol, PEG 8000 |
