6WKU

Twelve Chloride Ions Drive Assembly of Human alpha345 Collagen IV NC1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-14
Detector	RAYONIX MX300HE
Wavelength(s)	0.97857
Spacegroup name	P 41 21 2
Unit cell lengths	128.420, 128.420, 104.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.400 - 1.760
R-factor	0.1395
Rwork	0.139
R-free	0.16150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mpx
RMSD bond length	0.006
RMSD bond angle	0.896
Data reduction software	iMOSFLM (7.2.0)
Data scaling software	Aimless
Phasing software	MOLREP (11.5.04)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.600	1.790
High resolution limit [Å]	1.760	1.760
Rmerge	0.083	0.488
Rmeas	0.089	0.521
Rpim	0.031	0.182
Number of reflections	86926	4534
<I/σ(I)>	13.1	3.4
Completeness [%]	100.0	100
Redundancy	8.2	8.2
CC(1/2)	0.998	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	PEG 200, sodium chloride, tris