6WK2

SETD3 mutant (N255V) in Complex with an Actin Peptide with His73 Replaced with Methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	60.342, 175.977, 66.314
Unit cell angles	90.00, 92.58, 90.00

Refinement procedure

Resolution	36.650 - 1.760
R-factor	0.1824
Rwork	0.182
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mbj
RMSD bond length	0.005
RMSD bond angle	0.781
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.650	1.810
High resolution limit [Å]	1.760	1.760
Number of reflections	129205	11330
<I/σ(I)>	13.8
Completeness [%]	94.7
Redundancy	5.2
CC(1/2)	0.993	0.396

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	292	0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000