6WK1
SETD3 in Complex with an Actin Peptide with His73 Replaced with Methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.388, 175.531, 66.654 |
| Unit cell angles | 90.00, 92.36, 90.00 |
Refinement procedure
| Resolution | 36.000 - 1.890 |
| R-factor | 0.1954 |
| Rwork | 0.195 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mbj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.745 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 102262 | 8146 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 93.6 | |
| Redundancy | 4.7 | |
| CC(1/2) | 0.986 | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000 |
