6WJL

Crystal structure of Glypican-2 core protein in complex with D3 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033190
Spacegroup name	P 1 21 1
Unit cell lengths	54.942, 225.646, 107.250
Unit cell angles	90.00, 104.75, 90.00

Refinement procedure

Resolution	29.476 - 3.300
R-factor	0.2794
Rwork	0.278
R-free	0.31700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4acr
Data reduction software	XPREP
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.476	3.418
High resolution limit [Å]	3.300	3.300
Number of reflections	37835	3807
<I/σ(I)>	7.3
Completeness [%]	99.8
Redundancy	5.8
CC(1/2)	0.991	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M NaCl, 10% (w/v) PEG 3350 and 0.1 M disodium hydrogen phosphate