6WJ7

The structure of NTMT1 in complex with compound C2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97934
Spacegroup name	P 31 2 1
Unit cell lengths	72.804, 72.804, 80.131
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.590 - 1.420
R-factor	0.166
Rwork	0.165
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dtn
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.440
High resolution limit [Å]	1.420	1.420
Number of reflections	46403	4545
<I/σ(I)>	20.4	1
Completeness [%]	99.9	99.78
Redundancy	6.5
CC(1/2)	0.990	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.8 M Lithium Chloride 0.1 M Tris: HCl, pH 8.5 32 % (w/v) PEG 4000