6WI8
Inhibitor compound-induced confrontational change in Ring1b-Bmi1 domain structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-12-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.081, 154.742, 49.579 |
| Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
| Resolution | 41.721 - 3.092 |
| R-factor | 0.2119 |
| Rwork | 0.207 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wi7 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.541 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.090 | 3.100 |
| Number of reflections | 7292 | 7320 |
| <I/σ(I)> | 13.28 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277.15 | 100 mM acetate, pH 4.5, 200 mM lithium sulfate, 2.5 M sodium chloride |
