6WI2

Structure of human mitochondrial complex Nfs1-ISCU2-ISD11 with E.coli ACP1 at 1.95 A resolution (NIAU)2. N-terminal mutation of ISCU2 (L35) traps Nfs1 Cys loop in the active site of ISCU2 without metal present.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.987
Spacegroup name	P 41 21 2
Unit cell lengths	86.430, 86.430, 246.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.011 - 1.950
R-factor	0.1521
Rwork	0.150
R-free	0.18490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6w1d
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.011	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	69004	6795
<I/σ(I)>	22.62
Completeness [%]	100.0
Redundancy	20
CC(1/2)	0.999	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	288	0.1 M MES pH 5.5 22.5 % PEG 400