6WHE

Structure of phosphomimetic Rab8a GTPase (T72E) in the GTP-bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979279
Spacegroup name	P 1 21 1
Unit cell lengths	36.092, 118.377, 39.603
Unit cell angles	90.00, 101.96, 90.00

Refinement procedure

Resolution	29.290 - 1.730
R-factor	0.1888
Rwork	0.187
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lhw
RMSD bond length	0.006
RMSD bond angle	0.859
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.290	1.760
High resolution limit [Å]	1.720	1.720
Rmerge	0.135	0.441
Rmeas	0.154	0.510
Rpim	0.072	0.250
Number of reflections	144140	1551
<I/σ(I)>	6.9
Completeness [%]	96.0	85.2
Redundancy	4.4	3.9
CC(1/2)	0.986	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	289	20% PEG3350, 0.1M Hepes pH 7