6WH8
The structure of NTMT1 in complex with compound BM-30
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 106.867, 106.867, 205.431 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.550 - 1.729 |
| R-factor | 0.1804 |
| Rwork | 0.180 |
| R-free | 0.19990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e1d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.879 |
| Data reduction software | HKL-2000 (denzo) |
| Data scaling software | HKL-2000 (scalepack) |
| Phasing software | PHASER (within PHENIX) |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.550 | 1.760 |
| High resolution limit [Å] | 1.729 | 1.729 |
| Rmeas | 0.224 | 4.823 |
| Rpim | 0.069 | 1.516 |
| Number of reflections | 72963 | 3997 |
| <I/σ(I)> | 12.8 | 0.7 |
| Completeness [%] | 99.9 | 98.1 |
| Redundancy | 19.4 | 18.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Na-HEPES, pH 7.5, 1.6 M ammonium sulfate, and 2% PEG 1000 |
