6WGL
Dupilumab fab with Crystal Kappa design complexed with human IL-4 receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-28 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.490, 73.490, 412.441 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.820 |
| R-factor | 0.2481 |
| Rwork | 0.245 |
| R-free | 0.30370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wg8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.242 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 103.110 | 103.110 | 2.970 |
| High resolution limit [Å] | 2.820 | 8.920 | 2.820 |
| Rmerge | 0.140 | 0.056 | 1.055 |
| Rmeas | 0.143 | 0.057 | 1.079 |
| Rpim | 0.031 | 0.013 | 0.221 |
| Total number of observations | 617796 | 20556 | 92510 |
| Number of reflections | 28706 | 1096 | 4060 |
| <I/σ(I)> | 18.1 | 38.7 | 2.9 |
| Completeness [%] | 99.9 | 99.6 | 99.9 |
| Redundancy | 21.5 | 18.8 | 22.8 |
| CC(1/2) | 0.988 | 0.996 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 100mM Hepes pH 7.5, 25% PEG MME 550 |
