6WG4

Crystal structure of human SMC1-SMC3 hinge domain heterodimer in south-open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97926
Spacegroup name	I 2 2 2
Unit cell lengths	78.291, 119.612, 127.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.560 - 2.310
R-factor	0.1877
Rwork	0.183
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wd5
RMSD bond length	0.008
RMSD bond angle	1.052
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.053	1.108
Rmeas	0.055	1.180
Rpim	0.015	0.389
Number of reflections	21541	751
<I/σ(I)>	43.6	1.2
Completeness [%]	98.5	86
Redundancy	12.3
CC(1/2)	0.999	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	2% 1,4-dioxane, 0.1 Tris pH 8.0, 15% polyethylene glycol 3350