6WEO

IL-22 Signaling Complex with IL-22R1 and IL-10Rbeta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03317
Spacegroup name	P 1
Unit cell lengths	134.487, 145.219, 152.038
Unit cell angles	71.06, 81.84, 62.48

Refinement procedure

Resolution	48.090 - 2.600
R-factor	0.2367
Rwork	0.236
R-free	0.29630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dgc 3lqm
RMSD bond length	0.011
RMSD bond angle	1.323
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.090	2.670
High resolution limit [Å]	2.600	2.600
Rmerge	0.095
Rmeas	0.116
Rpim	0.066
Number of reflections	289478	14313
<I/σ(I)>	6.5
Completeness [%]	97.5
Redundancy	2.9
CC(1/2)	0.994	0.331

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	12.5% PEG 3350, 0.2 M sodium pottassium phosphate, 0.1 M HEPES pH 8.0