6WBC

Structure of Mouse Importin alpha- MLH1-R472K NLS Peptide Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-20
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.425
Spacegroup name	P 21 21 21
Unit cell lengths	78.697, 90.229, 100.077
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.114 - 2.150
R-factor	0.1817
Rwork	0.180
R-free	0.21260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u5p
RMSD bond length	0.004
RMSD bond angle	0.702
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.760	2.227
High resolution limit [Å]	2.150	2.150
Rmerge	0.086	1.753
Rmeas	0.086	1.822
Rpim	0.025	0.494
Number of reflections	514128	50867
<I/σ(I)>	24.4
Completeness [%]	99.8	99.36
Redundancy	13.1
CC(1/2)	1.000	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.1 M sodium citrate (pH 6), 0.575 M sodium citrate , 10 mM DTT