6WBB
Structure of Mouse Importin alpha - MLH1-E475A NLS peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-20 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.425 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.268, 90.556, 100.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.912 - 2.663 |
R-factor | 0.1869 |
Rwork | 0.183 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u5p |
RMSD bond length | 0.003 |
RMSD bond angle | 0.657 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.912 | 2.758 |
High resolution limit [Å] | 2.663 | 2.663 |
Rmerge | 0.151 | 1.122 |
Rmeas | 0.157 | 1.167 |
Rpim | 0.043 | 0.316 |
Number of reflections | 274753 | 27323 |
<I/σ(I)> | 19 | 2.49 |
Completeness [%] | 99.8 | |
Redundancy | 13.1 | |
CC(1/2) | 0.998 | 0.803 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1 M sodium citrate (pH 6), 10 mM DTT, 0.55 M sodium citrate |