6WAX

C-terminal SH2 domain of p120RasGAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	52.499, 65.588, 71.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.368 - 1.500
R-factor	0.1799
Rwork	0.179
R-free	0.19680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d4t
RMSD bond length	0.006
RMSD bond angle	0.872
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.095	0.073	1.026
Rmeas	0.099	0.076	1.106
Rpim	0.028	0.021	0.407
Total number of observations	471661
Number of reflections	40194	4270	3941
<I/σ(I)>	7.4
Completeness [%]	100.0	100	99.9
Redundancy	11.7	12.5	7
CC(1/2)		0.997	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2.3 M Ammonium Sulfate, 0.1 M Bis Tris pH 6.5