6WAV

Crystal structure of PHF1 in complex with H3K36me3 substitution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-08
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	153.003, 65.616, 29.026
Unit cell angles	90.00, 97.12, 90.00

Refinement procedure

Resolution	32.802 - 1.700
Rwork	0.197
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hcz
RMSD bond length	0.011
RMSD bond angle	1.664
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.070	40.070	1.730
High resolution limit [Å]	1.700	8.990	1.700
Rmerge	0.048	0.031	0.416
Rmeas	0.056	0.038	0.487
Rpim	0.029	0.021	0.251
Total number of observations	107846	706	5410
Number of reflections	29381	219	1501
<I/σ(I)>	16.4	34.3	3.1
Completeness [%]	93.8	94.5	90.9
Redundancy	3.7	3.2	3.6
CC(1/2)	0.998	0.998	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	1.2 M ammonium sulfate and 0.1 M sodium acetate 4.6