6WA8

Crystal structure of the E. coli transcription termination factor Rho

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	161.745, 101.902, 184.016
Unit cell angles	90.00, 107.77, 90.00

Refinement procedure

Resolution	39.790 - 3.300
R-factor	0.2537
Rwork	0.252
R-free	0.29610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pvo
RMSD bond length	0.002
RMSD bond angle	0.576
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.790	3.418
High resolution limit [Å]	3.300	3.300
Rmerge	0.206	1.430
Rmeas	0.223	1.551
Rpim	0.084	0.597
Number of reflections	43015	4146
<I/σ(I)>	8.92	1.34
Completeness [%]	99.6	97.32
Redundancy	7	6.6
CC(1/2)	0.995	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	293	100 mM NaCl, 100 mM Tris pH 8.3, 28% PEG2000MME, 20 mM ATP, pH 7.5