6W9D

RNF12 RING domain in complex with a Ube2d2~Ub conjugate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 31
Unit cell lengths	135.473, 135.473, 51.372
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.058 - 3.190
R-factor	0.2245
Rwork	0.222
R-free	0.28060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq
RMSD bond length	0.002
RMSD bond angle	0.527
Data reduction software	XDS
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.060	3.410
High resolution limit [Å]	3.189	3.190
Rmerge	0.088	0.638
Rmeas	0.114	0.827
Rpim	0.071	0.514
Number of reflections	17289	3172
<I/σ(I)>	8.2
Completeness [%]	98.3	99
Redundancy	2.3	2.3
CC(1/2)	0.995	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	15-20% PEG 3350, 200-400 mM NaI