6W8D

Structure of DNMT3A (R882H) in complex with CGT DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	.5
Spacegroup name	H 3
Unit cell lengths	205.560, 205.560, 89.505
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.159 - 2.598
R-factor	0.2015
Rwork	0.199
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yx2
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.160	2.690
High resolution limit [Å]	2.598	2.600
Number of reflections	43419	43403
<I/σ(I)>	13.7
Completeness [%]	98.9
Redundancy	5.1
CC(1/2)	0.998	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Sodium Citrate pH 4.2, 1% PEG8000