6W73

Fab Structure of CD4 Binding Site (CD4bs) Huamn Monoclonal Antibody HmAb64

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-30
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	91.790, 59.352, 75.571
Unit cell angles	90.00, 96.22, 90.00

Refinement procedure

Resolution	45.630 - 2.800
R-factor	0.1822
Rwork	0.178
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dee
RMSD bond length	0.008
RMSD bond angle	0.974
Data reduction software	XDS
Data scaling software	XDS (1.16_3549)
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.630	2.970
High resolution limit [Å]	2.800	2.800
Rmeas		0.530
Number of reflections	21339	1621
<I/σ(I)>	11.6	2.84
Completeness [%]	99.8
Redundancy	4.2
CC(1/2)	0.995	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	30% PEG1500, 8%MPD, 0.1M Tris pH8.5